Evaluation of responses to tomato brown rugose fruit virus (ToBRFV) and selection of resistant lines in Solanum habrochaites and Solanum peruvianum germplasm

Abstract Reactions of plants in 173 wild tomato accessions belonging to Solanum habrochaites and S. peruvianum were studied by inoculation with a tobamovirus, brown rugose fruit virus (ToBRFV). Around 10–50% nine one demonstrated be highly resistant. Resistant showed no symptoms at 22–24 °C, could detected their inoculated newly developed leaves using bioassays RT-qPCR. ToBRFV-resistant also resistant tobacco mosaic virus. The susceptible tomatoes infected systemically ToBRFV showing different severity symptoms. When incubated plant growth chamber temperature 33 they expressed deformation symptoms, indicating that the resistance was broken elevated temperature. However, when these transferred greenhouse 24 emerged not new leaves. Cleft grafting done scions from LA1739 into ToBRFV-infected rootstock. became ineffective after grafting. Sequences comparison Solyc08g075630 loci high heterogenity. Only carried an allele almost identical gene reported previously. All other may have probably unknown gene(s) ToBRFV.